FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1597703
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAT1454.6046625E-721.7272171
CACGAGC7150.021.53814570
GTGTATA3050.019.5109621
GTATTAT2555.4569682E-1119.218411
GTATTAA1852.2699533E-718.9215981
GTATAAG2659.458745E-1118.4931871
GTGTAAA5150.018.3521161
CGTAGAC1351.4116839E-418.1501543
GTTTAAA4500.017.8914241
TCTGTCG3550.017.7462168
AGGGGGG5100.017.15660970
TACTAGT1250.001761329416.8023822
GTACATA1807.1449875E-515.557761
GTACTAG1807.1449875E-515.557761
TATAAGT2051.2579654E-515.3680322
GTCTTAC3704.3655746E-1115.13727951
GTATAAA2553.897494E-715.1001791
GTATTAG2101.5801988E-515.0021261
CTAGTAC1400.00375201815.0016573
GTCTAGC3051.20999175E-814.9201481
Produced by FastQC (version 0.11.5)