FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2363549
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATATG2000.033.2496870
TCTGTCG5400.023.3331078
GTATAAT4300.021.166171
GATCCGA850.003788428120.5915221
GTCCGGA1601.185681E-619.6906411
GTACTAG3050.019.5113091
ACGATAT24850.018.87305670
GTACTAC2456.6211214E-1018.5743921
GATCGAT1351.4115151E-418.1510451
TGTCGCC6650.017.89456410
CTGTCGC6850.017.883049
ATCTCGT7050.017.87216870
CGCATAG1000.0096938517.49983212
GTCGCCC6400.017.4998311
ATTGCGT1200.001341723417.499838
GTCTCGC5500.017.184561
TATCTCG8150.016.31886170
GGTATAC3501.8189894E-1116.0025541
GTATAGG3501.8189894E-1116.0025541
CGTATAA1755.625353E-515.999845512
Produced by FastQC (version 0.11.5)