FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2213960
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG700.001226773525.0004925
TCTGTCG4800.024.7915948
GTCTCGC4700.024.5760611
GTATTAA4150.023.615991
GTATAGT3800.021.1855851
CGAACAA2650.021.13201370
GTGTAAA7000.021.0013641
CTGTCGC5550.019.5494929
TGTCGCC5800.019.3102910
GTATTAC3300.019.0921481
GTCGCCC5150.019.0290711
GTACGGA950.007209962218.4222471
TCGGAAC950.007210901518.4218333
GTATGAC2802.0736479E-1017.5007383
GTATAAC2702.413799E-916.8529431
GTATAGA3950.016.8365361
GTGTAAG7100.016.7616521
TCGCTCT5850.016.7524684
GTCTCAC14900.016.4440211
GTATTAG4900.016.4296361
Produced by FastQC (version 0.11.5)