FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1687463
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG3450.027.3910858
ATACCGT906.176768E-627.222816
GCGTTAT657.957128E-426.9252551
GTACGGA750.001832522323.335221
TCGCTCG952.781747E-422.1057415
GTATAAT2401.8189894E-1221.8767681
GTATAGA2900.020.51891
TATCTCG5650.020.44231470
GTATTAA3000.019.8349381
GGGTACG900.005275401819.4460161
TGTCGCC5100.018.52926310
GTCTCGC4550.018.463031
GGTACGG1351.4123846E-418.1490782
TCGCCCA5050.018.02019112
GTGTAAT3950.017.7229521
GGCGTAT1000.00968768817.5014131
TTACGGG1000.009692647517.49985910
CTCCGAC2259.407813E-817.11097370
GTGTAGG5350.017.0107211
GTATTGA3307.2759576E-1216.971071
Produced by FastQC (version 0.11.5)