FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2189689
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG5050.028.4153338
GTCTCGC5900.025.5126781
CTGTCGC5600.025.0001229
TCGCTCT5950.024.1199684
TGTCGCC5800.023.534610
GTACGGA1052.039121E-523.337181
TCTCGCT7050.021.8460732
CTCGCTC7650.020.5902183
GTATTAC3650.020.1403061
GCGCTAT900.005273334719.4476491
GTGTAAC4750.019.161051
GTCGCCC6050.019.09100311
GTATTAG4250.018.9442981
GTCCGGG2254.62569E-918.6697431
TATCTCG6500.018.30736470
GTATAAA5950.018.2382981
GTATAGT4800.018.232171
GTATAAG3750.017.7362561
GTATAAT4550.017.6952231
GTGTAAA6800.017.5028841
Produced by FastQC (version 0.11.5)