FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2075524
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG400.002419672235.0006144
GTATTAC3200.028.4386841
CTGCGTA851.3095752E-424.705729
TCTGTCG5050.023.5642038
CGGTAGT750.001833595923.3331816
CTGTCGC5850.022.7348949
TACACCG800.00266796521.8748575
GTGTCGA1003.9352782E-420.9998636
CGGTACT850.003791393720.5885964
CGCACGA850.00379192320.588114
GTCTTGC13000.019.116181
TGTCGCC6350.018.74003610
GTATATA5050.018.713651
GTATAGT3800.018.421821
GTCCGGA1150.001008108418.2616311
TCGCCCA6500.017.76911412
GTATTAT4150.017.711581
TGCGTAA1200.001341620817.49988610
GTCTCGC5300.017.1705251
TGTTGCG1452.4401837E-416.8968492
Produced by FastQC (version 0.11.5)