FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3019245
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13650.046.9138571
TCTGTCG13300.043.8425878
TCGCTCT16600.037.2970664
CCGATCG850.002838590633.8797159
TCTCGCT18850.033.2270852
CGCTCTG18350.032.563155
GTCTTGC27550.031.2592661
CTCGCTC18450.030.0454483
CTGTCGC22350.028.3467869
GTATTAG10450.028.1746331
GCGTTAC1709.31027E-525.9786661
GTGTTAG19850.025.215231
TATACGG1500.001557447423.997345
GTCTTAC11450.023.785421
GGGTTTC47650.023.0164281
TTGCTCT37400.022.7140584
GTTTCAC43950.022.4411963
GTGTAAT12300.021.5432851
GTCTCAC34600.021.486211
GTACTAC5151.8189894E-1221.4387071
Produced by FastQC (version 0.11.5)