FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2927294
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14200.038.6345331
TCTGTCG11650.033.9848448
TCGCTCT16100.033.5338864
GTCTTGC28600.032.7375871
GTATAAC5150.032.6680641
TCTCGCT16950.031.431312
CTCGCTC17650.029.3654253
GTATTAG10500.029.259221
CGCTCTG17500.026.7376885
GTACTAT5700.025.6659831
GTCTCAC35050.025.4609491
GTTTCAC43700.025.0386373
GTGTTAG20450.025.0384541
TTGCTCT36650.024.9446984
GTATTGC8650.024.5236261
CTTGCTC36400.024.3249663
ACCGTGT10200.023.995368
GTATAGG8350.023.6526641
GTCTTAC10550.023.5737821
CTGTCGC17500.023.4468969
Produced by FastQC (version 0.11.5)