FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2927294
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13550.048.3795131
TCTGTCG13750.040.8368238
TCGCTCT16150.039.6727564
TCTCGCT17400.036.4089662
GTCTTGC28600.036.0556071
CGCTCTG17900.035.7941365
CTCGCTC17900.034.185413
GTGTAAC9950.030.3509031
GTATTAC6600.030.1321871
GTACTAT5950.028.4722421
CTGTCGC20600.027.608469
TTCACCG8850.026.8438975
TTGCTCT39100.026.144874
GTCTTAC11850.026.1060851
GTTTCAC44750.025.2569853
GTCTCAC33800.024.624791
GTACTAC5450.024.32691
GTGTTAC12400.023.7601451
TAATACT8300.023.4185814
GGGTTTC50150.023.2058221
Produced by FastQC (version 0.11.5)