FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2980829
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14150.054.435278
GTCTCGC13600.042.97451
GTCTTGC29500.041.3574871
CTGTCGC20700.038.601639
TCGCTCT14850.037.3265044
TCTCGCT15850.035.4316372
CTCGCTC16050.034.5357363
GGTATAC5500.033.2075651
CGCTCTG16800.029.9945135
TTGCTCT39500.028.2479954
GTGTAAA19100.027.9221731
CTTGCTC38850.026.3117873
TCTTGCT40650.025.8593852
GTTTCAC47100.025.8296663
GGGTTTC51500.025.8180771
ACCGTGT10400.025.6106978
TTACACT14150.025.437044
TAAACCG2301.1748652E-625.0388955
CGTTTCG1751.3186217E-424.6811989
GTCTTAC10800.024.3522151
Produced by FastQC (version 0.11.5)