FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2911520
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12250.045.8360638
GTCTCGC15100.043.1077651
GTCTTGC26150.035.799811
TCGCTCT16200.035.5487024
TCTCGCT17550.034.8686682
CTCGCTC18300.031.8627093
CGCTCTG19950.029.2274485
CTGTCGC19550.029.0890249
GTATTAG9550.028.3361451
GTGTAAC9850.028.2156331
GTATTAA7650.026.7694231
GTGTTAG18550.025.6278321
GTATTAT8100.025.2822341
GTCTCAC35100.025.2127781
GTGTTAC13200.024.9333721
GTGTAGT17150.024.734681
TCTGTCA38250.024.654078
GTATAGA8600.023.8123361
GTATTAC6250.023.4041251
GGTATAC5350.023.2400781
Produced by FastQC (version 0.11.5)