FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3100745
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13200.044.719048
GTCTTGC26900.037.1685561
GTCTCGC13550.033.1513561
GTATTAC5900.030.6998921
GTCTTAC11750.028.9806981
CTGTCGC22150.028.9246969
TCGCTCT16050.028.7110144
GGGTTTC47850.027.1031631
TTCACCG9700.026.7223345
TTGCTCT41650.026.1039094
GTGTTAG19500.026.0083221
GTACTAG6700.025.9528661
TCTCGCT16400.025.9031012
GTGTAAC9100.025.477541
GTGTTAA11350.024.8953131
ACCGTGT11350.024.1012888
GTCTCAC34850.023.4922471
CTTGCTC40500.023.4672663
CTCGCTC18450.023.4152343
GTACTCG2802.36103E-723.2880611
Produced by FastQC (version 0.11.5)