FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3082275
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12450.050.057521
TCTGTCG13900.048.164998
TCGCTCT14500.041.708444
TCTCGCT15100.038.1439552
GTCTTGC29800.036.476681
CGCTCTG15900.036.2247625
CTGTCGC21200.033.956869
CTCGCTC16400.033.8033373
GTATAAT9700.029.8832991
TTGCTCT40050.028.0436654
GTACTAT7650.026.5238691
CTTGCTC37500.026.4947913
GTTTCAC47750.025.1800043
GGGTTTC52650.024.2244851
TAATACT9850.024.1207284
GTCTCAC37450.023.800911
GTGTTAC13500.023.6188741
GTGTAAA18750.023.575931
GTACGGA4053.274181E-1123.2610111
GTCTTAC11850.023.2383611
Produced by FastQC (version 0.11.5)