FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3101515
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12400.044.12288
GTCTCGC14550.040.8157161
TCGCTCT17100.037.475644
GTCTTGC25350.037.4256941
TCTCGCT17700.031.730472
CTCGCTC19150.029.7038973
CGCTCTG18200.029.2762535
GTACTAA6050.028.7297861
GTGTTAG19450.028.2989641
CTGTCGC21500.027.1217489
GTATTAT8950.026.7034531
TAATACT8600.025.9548424
GTTAGCC17500.025.9213523
TTCACCG8350.025.8696165
GTCTCAC35800.025.2873611
ACCGTGT9450.025.1393058
GTATTAG9400.024.6546381
TCCGTAC2051.3619927E-524.5866493
TTGCTCT38150.024.1585354
GGGTTTC50700.023.426771
Produced by FastQC (version 0.11.5)