FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3122396
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14500.046.4696041
TCTGTCG15450.039.138758
TCGCTCT17800.036.8084074
TCTCGCT17850.036.3019522
CGCTCTG18350.034.1357045
CTCGCTC18900.033.1423383
GTCTTGC30300.032.0417441
GTACTAC5700.027.9641651
GTATAGA9100.027.0702041
CTGTCGC22600.026.437839
GGTATAC6600.026.3464031
TTGCTCT39200.025.7138844
GTATATA12550.025.401711
GTATTAA9600.024.150871
GTATTAG10600.023.923031
GTATAAT9250.023.4981441
GTATACG2802.3607754E-723.2883381
GTCTTAC11950.023.0393221
GTGTTAG23550.022.7664241
TCTGTCA41650.022.29628
Produced by FastQC (version 0.11.5)