FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3195380
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13550.050.4699758
TCGCTCT16100.036.6717724
GTCTTGC30000.035.5048751
GTCTCGC15800.035.3122631
CTGTCGC21600.034.6599859
CTCGCTC18150.032.1330763
TCTCGCT18550.029.1112792
TTGCTCT40400.028.6938254
GTACTAG6350.027.3860531
CGCTCTG18900.027.0483285
TAGCCCG2456.893242E-826.4496735
GTCTCAC36600.025.5388051
GTTTCAC50550.024.9264723
CTGTTGC33400.024.785649
GGGTTTC55900.024.628261
TCTGTCA41350.024.372578
GTCTTAC11950.024.2540361
TCTGTTG34400.023.8558658
CTTGCTC40950.023.7368893
TTCACCG10050.023.6424125
Produced by FastQC (version 0.11.5)