FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3195380
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17250.039.2264678
GTCTCGC13600.034.3324361
GTCTTGC29950.032.398011
TCGCTCT15250.029.7402844
CTGTCGC24550.029.617229
GTGTTAC11500.026.6449571
GTATTAG9950.026.3962961
TACACTG18550.026.3900035
TAATACT9650.026.1104984
CGCTCTG17650.026.1041585
GGTATAC6450.026.0154721
GTTTCAC49250.025.4341643
GTCTCAC38350.025.3017081
AACCGCG1450.001279001524.8227127
GTATTAA9450.024.7048231
TTGCTCT42850.024.6968154
TCTCGCT17500.024.6828022
GGGTTTC53400.024.5920541
CTCGCTC17300.024.551633
TTACACT14700.023.996794
Produced by FastQC (version 0.11.5)