FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3759662
Sequences flagged as poor quality0
Sequence length150
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13800.046.4283648
GTCTCGC14050.039.199811
GTCTTGC29050.036.670591
CTGTCGC21800.031.7020389
TCGCTCT16900.031.5281414
TCTCGCT17850.027.4298882
CTCGCTC18450.026.1475963
TTGCTCT39200.026.0828824
CGCTCTG19050.024.946085
GTCTAGC12500.023.7695271
GTCTCAC31450.023.7335821
GAGTGCA89550.023.6393246
GGAGTGC84600.023.5755985
CTTGCTC37500.022.8490963
TCTTGCT44350.022.7293872
GTATAAT7550.022.0763651
CTGGAGT95200.021.858193
GTACCGA1750.003669932720.7051621
GTATTAA7350.020.7051621
GTATAGC6050.020.3629281
Produced by FastQC (version 0.11.5)