FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3480539
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15050.038.7458538
GTCTCGC16700.036.0038031
TCGCTCT18400.030.9130694
GTCTTGC30550.030.826121
TCTCGCT19550.028.3580782
CTCGCTC19650.027.4809363
GTCTCAC38600.026.6494221
TACACTA7900.026.43045
CTGTCGC22950.026.3495569
GGGTTTC57250.025.686641
CGCTCTG20200.025.3069525
GTGTAAC10250.024.7360821
GTATAAT9700.024.6450081
GTGTTAC13250.024.056011
TATACTG10000.023.0400185
GTTTCAC53800.023.0186043
TTGCTCT43850.022.823284
GTATTAC7700.022.5791341
GTACTAT7700.022.5791341
GTATTAT10750.022.2378221
Produced by FastQC (version 0.11.5)