FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3480539
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14550.040.0747648
GTCTCGC14950.037.074911
GTCTTGC31000.033.8772741
TCGCTCT16950.031.429844
CTGTCGC23550.028.7341529
CTCGCTC18050.028.7167593
GTATTAA9000.028.3617631
TCTCGCT19200.027.7490462
GTATAAT11100.026.93821
GTATTAT10600.026.8328291
GTGTTAC13850.026.328611
CGCTCTG18050.025.1271655
GTTTCAC52950.024.608933
GGGTTTC57250.024.4587021
TTGCTCT42650.023.9689754
GTATTAG10700.023.8556881
CGTTTCG2552.882245E-622.584549
GTATAAC5500.022.5420761
GTCTTAC12600.021.9948371
GTGTAAC10300.021.949881
Produced by FastQC (version 0.11.5)