FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3418826
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12300.040.3831638
GTCTCGC15450.039.369661
TCGCTCT16050.036.3369874
GTCTTGC29900.033.905311
GTATTAG9550.032.604381
TCTCGCT17500.031.6804712
CGCTCTG18100.030.6302875
GTGTTAG23600.028.8420891
CTCGCTC18400.028.174333
GTATTAC7750.027.0961231
GTATTAA9650.026.263441
GTATTAT9100.026.2593171
CTGTCGC19850.025.7486329
TAATACT9550.025.6338884
GTCTTAC12550.024.2335171
GTCTCAC36650.023.9068431
TTGCTCT43850.023.3161954
CGGTACT2502.4230249E-623.0403424
GTATAAC6350.022.8069461
GTTAGCC18000.022.8003373
Produced by FastQC (version 0.11.5)