FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3475661
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16050.044.4031878
TCGCTCT19100.040.7052734
GTCTCGC16550.040.540141
TAATACT8650.037.4504134
GTCTTGC31450.035.710471
CTCGCTC20600.033.5478173
CGCTCTG20250.030.9281865
TCTCGCT21200.030.2235622
GTCTTAC12600.028.9397581
CTGTCGC26500.027.9798479
GTATTAG10750.027.136071
GTTTCAC47300.026.7862423
TTGCTCT42850.026.3760074
GGGTTTC55000.025.723761
GTGTTAG23400.025.5560321
TTATACT10000.025.1958084
GTATAAT9400.024.8266181
TAGTACT7250.024.8234564
GTATAGA10600.024.7680641
GTCTCAC36800.022.9882341
Produced by FastQC (version 0.11.5)