FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2089108
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9450.055.618938
GTCTCGC10850.045.144861
CTGTCGC11750.043.506299
TCGCTCT11650.038.3165974
TCTCGCT11700.038.1674652
CTCGCTC12050.035.858273
CGCTCTG12300.035.7063875
GTCTTGC20550.035.052361
GTTTCAC33450.029.4950793
GGGTTTC36550.028.1823841
GTGTTAC8550.027.8020551
TTCACCG6650.027.0669615
TTGCTCT26050.025.9801274
GTGTAAC5900.025.6387231
GGTTTCA35200.025.3726922
AATACCG2001.1252028E-525.1993435
ACCGTGT6950.024.8632558
GTATAAT4950.024.7384681
GTATTAG7300.024.66871
GTATAGA6450.024.569261
Produced by FastQC (version 0.11.5)