FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2089108
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10200.037.981541
TCTGTCG9300.037.02868
GTCTTGC19950.035.242131
TCGCTCT12100.034.982084
CGCTCTG11750.031.7500515
CTCGCTC12450.031.6936573
GTGTTAC8000.031.3875241
CTGTCGC11350.030.3398889
GTCCTAC6100.029.4028321
TCTCGCT13450.029.337252
GGGTTTC34400.027.737811
GTGTAGT13350.027.4074021
GTCTTAC6750.026.5714471
GTGTTAG14750.026.2652021
TACACTG11550.025.467185
GTATTAT5700.025.1729531
TTGCTCT26200.024.9183394
GTTTCAC33550.024.591463
TAATACT6450.024.4704514
GGTTAAC4750.024.1660331
Produced by FastQC (version 0.11.5)