FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2872944
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11150.052.941218
CTGTCGC14150.044.2606439
TCGCTCT12950.041.190714
GTCTCGC13350.040.789671
GTCTTGC26100.039.7537731
TCTCGCT14400.038.548812
CTCGCTC15650.033.623733
CGCTCTG15600.031.8399095
TTGCTCT31800.030.1482324
GTGTTAC10200.029.5789951
CTTGCTC32150.028.0263393
GTTTCAC42200.026.8178863
GGGTTTC45350.026.7735771
GTCTTAC8850.025.7761551
GGGTACG2309.699725E-725.5953961
GTGTTAG19200.025.295451
TTCACCG8000.025.1950575
GCGTTAG2351.1699631E-625.0508141
GTACTAC4500.024.5289231
TCTTGCT37750.024.4441952
Produced by FastQC (version 0.11.5)