FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2872944
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13900.051.4951821
TCTGTCG14300.049.3386428
TCGCTCT14800.045.7260134
CTGTCGC19750.038.6398939
GTCTTGC27150.038.200921
CTCGCTC17350.036.5163963
TCTCGCT17500.036.20342
CGCTCTG17500.035.379985
TTGCTCT34200.033.260454
GTGTTAC11600.031.4823631
CTTGCTC35250.029.6151283
GTTTCAC38650.029.0589453
GTCTTAC10700.027.9869371
GGTATAC5750.027.9453891
CGTTTCG1306.7569345E-427.6900569
GGGTTTC42700.027.5393261
GTGTTAG17600.027.3896581
TCTTGCT38650.027.3824672
GTCTCAC30650.027.166251
GTACGAC1607.165193E-526.9982723
Produced by FastQC (version 0.11.5)