FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2218080
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9550.049.0209731
TCTGTCG9200.046.177298
TCGCTCT11650.042.0250324
GTCTTGC21050.037.978941
TCTCGCT11750.037.389042
CGCTCTG13450.035.3302545
CTGTCGC12450.034.7013329
CTCGCTC12800.034.3204273
GTGTCGC2752.3101165E-1028.809251
GTGTTAC9700.027.472741
TTGCTCT30250.026.895414
CTTGCTC28950.026.120023
GTGTAAA13650.025.8544541
TAATACT7000.025.7138484
GTGTATA12250.025.2815861
GTTTCAC34050.025.1688063
GGGTTTC37800.024.7698571
GTATAAC4101.8189894E-1224.5932621
GTATTAG9450.024.3887831
GTATAAA8400.024.0077081
Produced by FastQC (version 0.11.5)