FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2218080
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11500.041.1751251
TCGCTCT12200.033.519994
GTCTTGC21000.033.1391451
TCTCGCT14000.030.7476582
CTCGCTC13550.029.6508793
TCTGTCG10500.028.0145348
CGCTCTG13350.027.9454385
GGTATAC5700.025.1735251
GTGTAAC6050.024.9030631
GGGTTTC37450.024.5214981
TTGCTCT29300.024.2413274
GTGTTAG15900.023.4636231
GTACTAT5200.023.4549451
CTTGCTC27600.023.3949583
GTATTAG6750.023.3834061
TCATACG1550.001922836123.14349
GTGCGAC2252.8770217E-522.3205241
GGATACG1952.8082295E-422.0752431
CTGTCGC13400.021.9516879
GTGTTAC9500.021.9009651
Produced by FastQC (version 0.11.5)