FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2267261
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7800.040.6164258
GTCTCGC9950.036.19541
CTTATCG900.0037538132.000829
GTCTTGC20200.031.735481
TCGCTCT10750.031.479184
CTGTCGC10650.031.0993869
GTGTAAC7350.029.399531
GCGCTAA1255.3564034E-428.8115371
CTCGCTC12150.027.857483
TCTCGCT12200.027.745762
GTGTTAG16200.027.5665951
GTATTAT7450.026.1044141
GGTATAC4700.026.0529861
GTTTCAC33800.025.9934183
GTGTAAT9550.025.6437781
GGGTTTC39650.025.6142941
GTCTTAC9150.025.1904141
TTACTCT12900.025.1163674
GTCTCAC28000.023.6666221
GTACTAA4303.6379788E-1223.4512541
Produced by FastQC (version 0.11.5)