FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2267261
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11100.048.4749158
GTCTCGC11100.042.0115931
CTGTCGC13600.040.619129
TCGCTCT11550.038.5113264
GTCTTGC21650.036.119971
CTCGCTC13500.033.4800073
TCTCGCT13450.032.0042532
TTGCTCT30100.029.3168534
GTCTTAC8050.028.5189061
TACCCCG2904.474714E-1027.2128125
CGCTCTG15400.027.0200425
CTTGCTC30250.025.851153
GGGTACG2508.679126E-825.8274331
GTGTTAA10050.025.698941
TTACACT11750.024.4231054
TTATACT5900.024.3196184
GTGTAAC6200.024.3000051
GTATAGA7100.024.2511121
GTGTTAC10200.023.9142911
GTATTGA6300.023.9142911
Produced by FastQC (version 0.11.5)