FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1811086
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC7800.051.706971
TCTGTCG7950.051.6244548
CTGTCGC9400.047.4910249
TCGCTCT9350.043.1233184
TCTCGCT9050.039.7891922
GTCTTGC16150.039.243331
GTATTAT5400.036.0102121
TTACGCT1201.00324705E-536.000274
CTCGCTC10650.033.80683
CGCTCTG10150.032.6307875
GTGTTAG12550.030.4149991
GTTTCAC26400.029.7307783
GTCTTAC6900.029.2256771
GGGTTTC29000.027.5664371
CTTGCTC22900.027.3569373
TTCACCG5550.027.2434465
TGTACCG1358.4183755E-426.6668665
TTGCTCT23450.026.4053154
GTATAAC3306.730261E-1126.1892431
GTATTAG5800.026.076361
Produced by FastQC (version 0.11.5)