FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1811086
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7550.049.409028
GTCTCGC8550.048.6644331
GTACTAG3500.040.99321
GTCTTGC16000.039.9043161
CGCTCTG9950.038.933245
TCGCTCT9300.038.568874
CTGTCGC10100.037.644749
TCTCGCT11050.034.4083182
CTCGCTC11650.033.8677673
GTCTCAC20950.033.2152521
AGTACCG950.00497901830.2055135
TTGCTCT21250.029.7080124
ACTGTCG2452.2100721E-929.2808538
TCTGTCA23150.026.9598888
TCTCGAT7500.026.7822237
CTGTTCG2156.721566E-726.6932439
TCACTCT25950.026.26254
GTCTAGC6850.026.1817861
GTTTCAC26900.025.8683833
GTATTAC4450.025.793471
Produced by FastQC (version 0.11.5)