FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2067370
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9200.047.7422878
GTCTCGC9500.039.422671
CTGTCGC11600.039.1060379
GTCTTGC18550.038.4377141
CGCTCTG9600.036.748885
TCGCTCT10300.035.6493954
TCTCGCT11250.033.930452
CTCGCTC11600.030.4180133
GTTTCAC28850.029.2033583
GTGTTAC7700.028.9959451
GTCTCAC22950.027.9301741
TTGCTCT26100.027.5853634
GTTCTAC5450.026.4301591
CTTGCTC24600.026.345123
GTGTTAG12700.025.519671
GGGTTTC32850.024.9941
ACCGTGT7550.024.7963458
TTACACT8150.024.7354414
ACACCGT4101.8189894E-1224.5846146
GGTTTCA29650.024.5336952
Produced by FastQC (version 0.11.5)