FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2067370
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9600.048.573658
CTGTCGC12100.043.280889
GTCTCGC9350.042.1997341
TCGCTCT9650.038.6575784
GTCTTGC18000.036.2683261
TCTCGCT11550.032.9194452
CTCGCTC12050.030.3627953
CGCTCTG13250.029.237255
TTGCTCT23700.027.5455634
GTCTCAC22400.027.5428621
GTTCTAC4750.027.1855131
TAATACT5600.026.902334
CGGTACT1358.599217E-426.5702024
CTCGATC8300.025.9299568
TTATTCG1400.001063259725.6212674
GTCTTAC7350.025.3772531
TTCACCG5150.025.0740155
TCTGTTG20150.024.9219288
TCGATCT8400.024.7672239
TTACTCT9900.024.6378234
Produced by FastQC (version 0.11.5)