FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1834767
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC3550.038.5457951
TCTGTCG7400.037.9460268
GTCTTGC15300.036.245251
GTACGGT1604.8697984E-836.009891
GTCTCGC8650.034.969141
CTGTCGC9150.033.8361369
GTGTTAC6500.032.13191
GGTATAC3550.030.4308911
GTTTCAC24500.029.9796583
TCGCTCT9950.029.6684044
GGGTTTC30300.028.2849961
TTGCTCT22750.028.1670914
GTATTAT4700.027.5820431
GTATAAC2654.8985385E-927.1772751
TCTCGCT10600.027.1757932
GTCTCAC21500.026.4630811
GTGTAAT6900.026.0941221
TACACTA3109.313226E-1025.548445
GGTTTCA28500.025.5214062
GTCTAAC2851.0631993E-825.2700981
Produced by FastQC (version 0.11.5)