FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3034165
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT32850.10826701909751119No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11450.043.380088
GTCTCGC13950.036.4135551
GTCTTGC24350.035.373261
CTGTCGC15850.033.154349
TCTCGCT16100.032.6912352
TCGCTCT15900.032.644144
CTCGCTC16650.029.874783
TTGCTCT30900.028.462384
CGCTCTG16250.027.4653475
GTCTCAC26700.026.1939241
TCTGTTG26950.024.3068898
CTTGCTC31300.023.7226263
GGAGTGC72500.023.6311975
GAGTGCA76650.023.5726456
TCTTGCT35350.023.2514362
TCACTCT31950.023.2400044
CTGTTGC26900.023.0140449
GTGTAAA11000.022.7548751
TCGAACT19950.022.7323539
GGCTGGA97700.022.1534291
Produced by FastQC (version 0.11.5)