FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2960493
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12850.046.501748
GTCTCGC13600.036.5211451
GTCTTGC27050.036.4536361
CTGTCGC18550.035.7057469
TGTACCG2302.910383E-1134.431755
TCGCTCT14500.033.7625434
GTCTTAC11950.031.1728571
TTGCTCT35200.029.8610044
GTTTCAC44000.028.1434523
GTATAGG6050.027.768141
GTCTCAC32650.027.7404081
CTCGCTC16450.027.5725083
GTATTAA7850.026.9837721
TCTCGCT17150.026.8696142
GTGTTAG17950.026.8567731
GTGTAAA14650.026.4248541
GGGTTTC48100.026.2709241
CGCTCTG16750.025.7887735
GTTAACG1400.001041556425.7124543
GTGTAAG14250.025.1162951
Produced by FastQC (version 0.11.5)