FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2484748
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10300.044.7370038
GTCTCGC11350.038.0686041
TCGCTCT11950.037.9604844
CTGTCGC12750.036.140489
TCTCGCT12500.033.986082
GTCTTGC21650.033.927651
CTCGCTC13450.031.5855773
GTATTAT6400.030.380531
GCGTTAA1204.2232263E-430.005461
GGGTTTC41500.029.1522921
GTTTCAC37800.029.1446423
CGCTCTG13950.028.9049955
GGTATAC4550.026.9059941
GTATAGA7250.026.818671
TTCACCG7950.026.265765
GTGTTAC9000.025.6046581
GTATTAG9050.025.4631961
TTACTCT11100.025.2988474
GTCTTAC9700.025.2417051
GTGTAAA13750.025.139121
Produced by FastQC (version 0.11.5)