FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3059195
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14800.056.4229628
GTCTCGC15000.051.0275731
CTGTCGC18550.048.1213079
TCGCTCT15450.046.6543664
TCTCGCT17200.041.4925542
CGCTCTG17800.039.6331525
CTCGCTC17400.039.3545653
GTCTTGC29200.037.554871
GGGTTTC47100.029.3764821
GTCTCAC33050.028.2810211
ACCGTGT9200.028.1691868
TTGCTCT38200.027.926674
GTGTTAC12450.027.1926361
GTTTCAC42800.026.6093393
CTTGCTC37100.026.4232223
GTATTAT8700.026.2243841
TAGTACG1658.762835E-526.2112714
GTGTTAG19700.026.1513821
GTCTTAC11600.026.0128971
GTGTAAT10650.025.5690731
Produced by FastQC (version 0.11.5)