FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3114356
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12600.050.278148
TCGCTCT14400.045.54144
GTCTCGC14000.042.0439071
CTGTCGC15500.039.477929
CTCGCTC16800.036.0327573
TCTCGCT16150.035.6986662
GTCTTGC28150.034.7627681
CGCTCTG18800.032.930155
GTGTAAC10150.031.8953761
GTCTCAC34350.028.9166581
GGGTTTC46400.026.1641771
GTGTAAT12250.025.8269711
GTATTAA7800.025.4689061
TAACACT14750.025.4061494
GTGTTAC13250.024.988361
GTTTCAC44600.024.3988653
CTGTTGC32450.024.1812559
TGTACGG2101.6303862E-524.0222242
TTGCTCT40800.024.021844
GTGTTAA11550.022.933041
Produced by FastQC (version 0.11.5)