FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3114356
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13800.049.040918
GTCTCGC13050.043.6368641
GTCTTGC28450.042.5985951
TCGCTCT14850.037.81624
CTGTCGC19700.034.353539
TCTCGCT16450.032.392042
CTCGCTC16300.032.243713
GTGTTAG19550.030.9956511
TTGCTCT40100.028.5471324
CTTGCTC39650.027.9632243
GTCTCAC34750.027.7324711
TCTTGCT42600.027.2137762
GTTAGCC15150.027.087693
GTTTCAC44200.026.5506443
CGCTCTG16750.026.2195225
GGGTTTC49600.026.0531981
GTATTAG9000.025.9583381
GTATTAA8350.024.4816681
GTGTTAC12500.023.946571
CTCGATC13200.023.4533168
Produced by FastQC (version 0.11.5)