FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2834713
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11500.056.96478
GTCTCGC13200.049.0344771
TCGCTCT15400.044.003824
CTGTCGC15250.043.90119
TCTCGCT16050.039.0810132
CGCTCTG16450.038.510495
CTCGCTC17200.036.045683
GTCTTGC25250.035.829151
GTTTCAC43000.031.8543223
GGGTTTC47050.030.9526881
CCGTTCG1652.524077E-630.5405189
ACCGTGT9600.029.9951488
GTCTCAC32750.027.848591
TTCACCG8600.027.6234425
GTGTAAC7500.027.4593051
GTGTTAG19800.027.1175521
TTGCTCT33450.026.9399724
TTACACT12350.026.2681064
ACCATGT33750.026.022468
GTCCGGA2858.5037755E-925.807621
Produced by FastQC (version 0.11.5)