FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2801875
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11800.051.7341
TCGCTCT13600.045.5825924
TCTGTCG11850.044.3475958
TCTCGCT14600.038.5148122
CTCGCTC14800.037.990273
CTGTCGC15100.036.7095769
CGCTCTG15300.036.229075
GTCTTGC25800.032.2135621
GTATAGA7200.028.60261
GTATTAG7950.026.8293951
GGGTTTC41000.026.7289831
GTATTCG2009.538484E-625.7423421
GTTTCAC38100.025.1632163
TTGCTCT32400.025.1404444
TTCACCG7550.024.790445
TACCCCG2651.4759826E-724.4486275
GTCTCAC31950.024.1712131
CCTATCG1801.5960161E-423.9963029
CTGTTGC30100.023.9165829
GTGTTAC11400.023.8713461
Produced by FastQC (version 0.11.5)