FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2801875
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12500.048.4753151
TCTGTCG12650.045.532238
TCGCTCT14850.042.665394
GTCTTGC22800.041.6256521
CTCGCTC15750.036.1132053
TCTCGCT16350.035.667382
CGCTCTG16600.034.6977545
CTGTCGC16800.033.427579
GGTATAC4500.032.4466631
TTGCTCT33500.027.2947034
GTTTCAC37950.026.1810343
GTGTTAG16050.025.9270061
GTATTAC6500.025.8325371
GTCCTAT7350.025.8248981
GAGTGCA73950.025.4110016
GTATTAA7200.025.3489571
GGAGTGC67950.025.2177875
ATGGCGT13150.025.1855566
CTTGCTC33400.025.0052383
TAACACT13400.024.7156774
Produced by FastQC (version 0.11.5)