FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3206355
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13050.049.6463558
CTGTCGC15250.042.4842579
GTCTCGC14500.040.6048471
TCGCTCT16000.036.9493374
TCTCGCT16050.034.5898632
CTCGCTC15700.033.5224723
GTCTTGC31550.031.9578171
GTATTAG9900.030.4792731
CGCTCTG17850.028.229845
GTGTTAT11800.028.0663811
GTCTTAC11350.027.8822941
GGGTTTC51250.026.5664651
GTGTTAG21900.026.2123781
GTTTCAC45050.026.0858883
TTGCTCT39250.026.0832354
GTGTTAC12050.025.6518171
GTCTCAC35000.025.4432891
GTATTAC6950.024.3556061
GTTAGCC17800.024.3021143
CTTGCTC38700.023.6594983
Produced by FastQC (version 0.11.5)