FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3206355
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13900.047.651848
GTCTCGC13700.046.8948441
TCGCTCT14700.039.1813434
CTGTCGC18650.035.9013529
GTCTTGC31000.032.9707871
CTCGCTC18250.031.5597673
TCTCGCT19400.031.1758352
CGCTCTG16900.029.8207115
GTATTAG9400.029.5133631
GTCTTAC11900.029.44811
TAATACT9700.028.9467334
GTGTTAG18850.028.6604751
TTGCTCT40700.027.7723054
GCGTAGT3503.6379788E-1227.116791
GGGTTTC47500.027.0509191
GTATTCG2456.026676E-826.8188061
TATACGG1358.4192544E-426.667162
GTTTCAC43850.026.105633
GTGTAAG14600.026.0024011
GTGTAAT12250.025.6268561
Produced by FastQC (version 0.11.5)