FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2926675
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC26700.042.909581
TCTGTCG12950.042.2525638
GTCTCGC13500.041.621991
TCGCTCT13950.040.772014
GGTATAC5300.034.4215161
TCTCGCT16650.033.2966542
CTCGCTC16600.032.962093
CGCTCTG16000.032.8482445
AGTACCG1601.9838317E-631.4983145
CTGTCGC19450.031.093459
GTCTTAC10150.030.914931
GTATTAG7800.030.8734531
GTCTCAC31750.030.3386381
GTGTTAG17450.028.8548981
GTGTTAT9000.028.378631
TTGCTCT35700.028.0321144
TCTATCG1607.1647286E-526.9985548
GGGTTTC41000.026.6976661
GTTTCAC39400.025.947853
GTACTAG4950.025.0616471
Produced by FastQC (version 0.11.5)