FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2063692
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9350.041.5878071
TCTGTCG9550.041.4648448
TCGCTCT10800.035.334944
TCTCGCT11450.032.071332
GTCTTGC18200.032.0477451
CTGTCGC13600.031.7638479
CGCTCTG11300.031.2226625
CTCGCTC11700.030.7706363
GTATAGG6100.028.3312191
GTCTCAC22750.028.1703641
CTTGTCG1804.9643295E-627.9992439
GTACCGC1358.41333E-426.669821
GTATTAT5150.026.5662481
GTACCGG1907.548726E-626.5294551
TTGCTCT22850.026.1543664
GTGTTAC7300.024.6604521
TCACTCT25250.024.523894
GTATTAC4700.024.5135381
GGTATAC3851.8189894E-1124.3145641
GTTTCAC28450.024.0432763
Produced by FastQC (version 0.11.5)