FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2238967
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8350.056.0470738
GTCTTGC20200.043.4951931
GTCTCGC10850.040.488611
TCGCTCT11300.038.872764
CTGTCGC13200.035.4540259
CGCTCTG13150.032.856365
TACCCCG2206.712071E-1032.7319075
TCTCGCT13300.031.4029432
CTCGCTC14000.030.8615113
GTATTAT6200.030.2005251
GTGTAAA11950.030.1324791
GTCGTAC1453.6554462E-529.7999841
GTATAGA6150.029.2750511
TTGCTCT26200.028.0345044
GTATAGC4650.027.8774031
GTTTCAC31400.026.6024933
GTATAAC3255.638867E-1126.5907551
GTCTCAC25200.026.2917861
TGTACCG1658.820559E-526.1855265
CTTGCTC29100.025.7356033
Produced by FastQC (version 0.11.5)