FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2238967
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9050.043.781161
TCTGTCG10150.042.5632938
GTCTTGC18350.038.0810431
CGTTATA1201.0023596E-536.005472
TCGCTCT11200.034.7133564
GGCGTAC1851.7396269E-731.1523911
TCTCGCT11800.030.513112
CTGTCGC14400.030.001219
GTATTAG5800.029.8096161
GTTTACG1703.1671825E-629.663491
CGCTCTG13350.029.662135
CTCGCTC12200.029.507413
GTCTTAC8100.029.3495921
GTGTTAT7400.029.2053681
GTTTCAC31000.028.3347283
GTGTTAG14150.027.4922621
TTATACT5250.027.4278374
GTATTAA5550.027.258341
GTGCGGA3750.026.8948971
GGGTTTC35000.026.5518511
Produced by FastQC (version 0.11.5)